[Histonet] In-situ film zymography

[Dave]

Has anyone tackled the procedure in the subject line?  We are having difficulty of lifting the frozen tissue sections off the film after incubation and staining with comassie blue.  We then tried mounting the sections to slides instead of the film and laid the film on top of the slides but that was unsuccessful.  Any suggestions would be greatly appreciated.

Dave McClister, HTL
Medical University of South Carolina
Division of Cardiothoracic Surgery
114 Doughty St
Charleston, SC 29425
843-876-5178