[Histonet] Pecam-1 (CD31)

[Patsy Ruegg]

does anyone know of a rabbit cd31 or of an anti-goat labeled polymer
detection reagent.  my mouse livers have too much endogenous biotin even
with very aggressive a/b block to use an avidin/biotin detection system.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Angela
McNabola
Sent: Tuesday, January 20, 2004 10:08 AM
To: Loralee_Gehan <@t> URMC.Rochester.edu; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] Pecam-1 (CD31)






Hi,

We use Santa Cruz PECAM-1 M-20 (SC-15060) goat polyclonal.

We steam for 30 minutes in Dako TRS (S1699) for 30 minutes (cool to room
temperature).

We also avidin/biotin block (Vector), and use ABC (Vector elite kit).

Works great, especially on mouse xenografts.

Let me know if you need more information.

Angela McNabola, MS, HT(ASCP)SLS
Bayer Corp.
West Haven, CT


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