[Histonet] cryostat sections of undecalcified tissue implants

[Patsy Ruegg]

Liz,
It depends on how big and crunchy it is.  Dabbs method for cutting fs of
bone marrow biopsies undecalcified worked pretty well on small samples.  He
embedded in (I can't think of the name of it off the top of my head) but it
set up harder than OCT.
I have done whole rat femurs without decal using the tape transfer system
and D profile tungsten carbide knives.  I have also done fs on osicles of
collagen sponges treated with growth factors to stimulate bone and cartilage
formation.  Some of them at early stages could be cut in the cryostat
without using the tape and the more developed one's could not.
Hopes this helps.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Elizabeth
Chlipala
Sent: Monday, January 26, 2004 12:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cryostat sections of undecalcified tissue implants



Hello all

Is it possible to section undecalcified cryostat sections from implants
(no plastic) from a rat subcutaneous implant animal model.  The implants
will have possibly new bone and cartilage, not very dense bone, but some
bone.  Would I need to use a tape transfer system or could I use a
tungsten knife and be o.k.  Any suggestions would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com

Ship to Address:
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University of Colorado
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Boulder, Colorado 80309

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