[Histonet] long term storage of B-plus fixed bone marrow smears

Sharon Cooperman scoop <@t> mail.nih.gov
Thu Jan 22 21:29:24 CST 2004


I have some bone marrow smears that I would like to fix in B-plus and 
then store.  I'm thinking that my best option would be to fix the 
smears, then dehydrate them thru ethanol to xylene and then let them 
dry for storage.  Before staining (I would be doing 
immunhistochemistry or immunofluorescence) I would rehydrate the 
smears to water.  Has anyone ever stored bone marrow smears fixed in 
B-plus, B-5 or formalin long term and how did you do it, or does 
anyone have any advice?

Thanks,
Sharon
-- 
Sharon Cooperman




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