[Histonet] DAB precipitates Help please

[Anila Syed]

Dear All,

I have been using DAB successfully for a while, but today when I stained my
sections, there were huge clumps of DAB precipitates stuck all over the
section. I didn't do anything different and it was a brand new kit. I am
sure that I mixed it well as I vortex for about ten seconds prior to use.

Is there a way of removing these to see the section underneath? I have tried
copious washing with PBS and left them for a long time in alcohols and
histoclear.
All help gratefully appreciated,

Anila Syed


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