[Histonet] Rat Micronuclei scoring

[Elizabeth Chlipala]

When we did this in the past we used a diff quick stain to stain the
slides.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
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Boulder, Colorado 80309
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
ames1 <@t> breathe.com
Sent: Thursday, January 22, 2004 2:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rat Micronuclei scoring

Hi there,
I'm hoping someone can help me with a staining technique to view
micronuclei 
in PCEs and NCEs on rat bone marrow smears. Apparently the best
technique is 
the H&E stain but when I try it the haematoxylin is too strong. I have
tried 
reducing the concentration to 0.5% but the some of the PCEs are still 
blacked out. Am I doing something wrong? 

I have resolved the Eosin being overpowering by using aqueous Eosin. 

I hope everyone is enjoying the New Year. 

My thanks to anyone who can help, 

Amy Greenhalgh 

Scientist
Sequani Ltd,
Bromyard Road,
Ledbury,
HR8 1LH 



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