[Histonet] Rat Micronuclei scoring

[ames1 <@t> breathe.com]

Hi there,
I'm hoping someone can help me with a staining technique to view micronuclei 
in PCEs and NCEs on rat bone marrow smears. Apparently the best technique is 
the H&E stain but when I try it the haematoxylin is too strong. I have tried 
reducing the concentration to 0.5% but the some of the PCEs are still 
blacked out. Am I doing something wrong? 

I have resolved the Eosin being overpowering by using aqueous Eosin. 

I hope everyone is enjoying the New Year. 

My thanks to anyone who can help, 

Amy Greenhalgh 

Scientist
Sequani Ltd,
Bromyard Road,
Ledbury,
HR8 1LH