[Histonet] embarrassing question

[Geoff McAuliffe]

HI Sharon:

    In my experience the damage, if any, will depend on the tissue (some 
are more sensitive than others) and how good a look you get at it. 
Morphology that may be fine for the light microscope may not be fine for 
the EM.
    Cheer up, everyone I know has done this at least once!

Geoff

Sharon Cooperman wrote:

> We recently had someone perfuse mice with 0.2M Sorensen's PBS instead 
> of 0.1M (with 4% formalin).  I would expect the cells to shrivel, but 
> when I looked at H&E's of the sections they didn't look any worse than 
> some of our other blocks which were done correctly.  Does anyone know 
> what happens when you perfuse tissues with 2x Sorensen's PBS or have 
> experience with this problem to tell me what problems I should look 
> out for - eg. damage to the tissues that might not be obvious on a 
> quick scan of an H&E?
>
> Thanks,
> Sharon


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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