[Histonet] embarrassing question

[Sharon Cooperman]

We recently had someone perfuse mice with 0.2M Sorensen's PBS instead 
of 0.1M (with 4% formalin).  I would expect the cells to shrivel, but 
when I looked at H&E's of the sections they didn't look any worse 
than some of our other blocks which were done correctly.  Does anyone 
know what happens when you perfuse tissues with 2x Sorensen's PBS or 
have experience with this problem to tell me what problems I should 
look out for - eg. damage to the tissues that might not be obvious on 
a quick scan of an H&E?

Thanks,
Sharon
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Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-7735
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