[Histonet] glycol methacrylate and sectioning

[Geoff McAuliffe]

Hi David:

    If you want 30 micron sections of brain why not frozen sections? or 
celloidin sections? I have never heard of cutting glycol methacrylate 
that thick, I always thought the purpose of plastic sections was thin 
sections.  Perhaps the "plastic" people on this list can provide advice.

Geoff

David Laidley wrote:

>This may be a stupid question but can a sliding microtome cut brain tissue at 30 um when embedded in glycol methacrylate. I know that there exists rotory microtomes that cut glycol methacrylate but I can't seem to find any information about sliding microtomes.
> 
>If you can cut 30 um sections of brain tissue in glycol methacrylate then does anyone have any tips on how this might be done (well). Or at least any tips or tricks you may have picked up over the years that may make the process go more smoothly.
> 
>David Laidley (MSc student)
>Memorial University of Newfoundland
>Division of Basic Medical Sciences, Neuroscience
>dave_laidley <@t> yahoo.ca
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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