[Histonet] PAS with and without Diastase for glycogen

[Geoff McAuliffe]

Hi Tammy:

    So the diastase is the problem? Order a fresh bottle from Sigma. I 
have always had good results with their alpha amylase, 0.25% in 4mM 
sodium acetate buffer, pH 6.0 for 10-15 min at room temp. This is on 
liver (rat or mouse) fixed 48 hours in neutral buffered formalin. I 
found no need to warm the enzyme sol'n to 37 degrees.

Geoff

Barnhart, Tammy wrote:

>We have been having problems with this stain for sometime.  It has worked
>hit and miss. The Schiff's reaction seems to be fine.  Shouldn't most any
>liver control work?  We have tried everything, new Schiff's, new controls,
>spitting, amylase...I'm outta ideas here.  Could you please advise...any and
>all help would be appreciated.  What am I missing???
>
>Tammy Barnhart
>
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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