[Histonet] folds forming in bone/cartilage sections
Mike Sohaskey
sohaskey <@t> socrates.Berkeley.EDU
Wed Jan 14 12:36:40 CST 2004
Hello,
I find that, when sectioning EDTA-decalcified mouse tibiae at
5 - 10 microns, that folds (i.e., creases) frequently form
specifically within the growth plate cartilage as the sections dry
and adhere to the Superfrost slides. I have had no success with
increasing the amount of time that I float the sections on a water
bath prior to drying them on slides. I'm hoping that someone might
be able to offer a suggestion as to how I can overcome this
difficulty. Thanks very much.
MIKE
Michael Sohaskey, Ph.D.
University of California, Berkeley
Molecular and Cell Biology
585 Life Sciences Addition
Berkeley, CA 94720-3200
sohaskey <@t> socrates.berkeley.edu
(510) 643-2775 (phone)
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