[Histonet] mouse bile duct epithelium

Jan Shivers shive003 <@t> umn.edu
Thu Jan 8 16:27:49 CST 2004

I have used a rat monoclonal against mouse cytokeratin CK8 (clone TROMA-1;
from U of Iowa Hybridoma Bank).  It stained well, but at the dilution
recommended (1:2) I felt there was nonspecific background as well.  With
more of a work-up, this could probably be improved.

In the following article you will find other mouse CKs listed and their
sources-  Spontaneous mouse mammary tumors: incidence and cytokeratin
expression; Research in Veterinary Science; 1997; 63; 85-89.

Jan Shivers
U of MN Vet Diag Lab

----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t> colobio.com>
To: "Histonet <@t> Pathology. Swmed. Edu" <histonet <@t> pathology.swmed.edu>
Sent: Thursday, January 08, 2004 2:24 PM
Subject: [Histonet] mouse bile duct epithelium

> here is my story:
> i am trying to label ms bile duct epithelium with mouse anti-mouse
> cytokeratin 7 and 19 (sounds odd, but that is what the investigator tells
> it is, not even rat anti-mouse) they tried the anti-human ck7&19 and it
> not cross react with the mouse.  I have the reagents to biotinylate these
> ms<ms antibodies but in my experience with these liver samples endogenous
> biotin is a huge problem even with the most aggressive A/B blocking.  Is
> there another possible rabbit antibody I could use that would label ms
> duct epithelial cells?  Would factor VIII, cd31, or cd34 stain these
> I know that the previous mentioned markers are for endothelial cells but I
> am just hoping they might work?????  I have not been able to find any
> say rabbit anti-ms ck7 or ck19 out there, has anyone else.  How about the
> new rabbit monoclonals, is anyone making ck7/ck19?  By the way I have
> and ffpe bile duct samples, so I can use either.
> Please advise.
> Patsy Ruegg
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