[Histonet] frozen heart again
pruegg <@t> colobio.com
Thu Jan 8 14:39:17 CST 2004
I have pieces of frozen human heart that was not optomally frozen (meaning
they just put it in a -70 freezer without cryo protectant OCT or anything).
I cut off a piece while it was still frozen and mounted some in OCT and made
frozen sections. As you can imagine the sections are not good. The muscle
is shredded looking with lots of spaces and unrecognizable as muscle. There
are cells which could be myocytes but who knows. I did some IHC on them and
definately have nuclear envelope staining on some cells. I am labeling with
Lap2 and Lamin A&C. I also tried Ki-67 and CD117-cKit, these were both
completely negative (good neg. control I guess). Negatives were negative.
Is it possible to reserrect this frozen tissue or will it always be ruined?
If I placed it in formalin and processed it in paraffin what do you suppose
it would look like. These antibodies seem hardy and may work on ffpe
tissue. These people are doing dna studies on these frozen heart samples
and are not concerned about preserving the tissue and cell morphology.
Please provide me with some wisdom here. Thank you.
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