[Histonet] Iron stain

[Tracey Smith]

Rebecca,  in most labs I've worked in, we use a Pearle's Prussian 
Blue method for all our Iron stains regardless of tissue or blood smear. You do need to fix the smear in Acetone free methanol for fifteen minutes first. If you are looking for siderocytes, then the preferred method  would be a Dacie stain with fixing the smear first in 37% formaldehyde fumes for at least five minutes.  Since I am now at home I do not have the methods handy but I would guess most good staining books would have the procedures in them.  Signing off from the frozen tundras of Minnesota.  Tracy Smith(proud member of SOW'96)
>>> "Rebecca Barnhart" <RBARNHART <@t> summithealth.org> 01/07/04 01:36PM >>>
What stain does anyone use for bone marrow aspirate, blood smears and
tissue.  Also how long does it take to do the entire stain.  We
purchased a kit and when we try to stain a fixed blood smear everything
is washed away.  Thanks in advance.

Becky Barnhart
rbarnhart <@t> summithealth.org 

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Tracy Smith
Anatomic Pathology Scientist
Children's Hospital and Clinics
Phone(651)220-6561
Fax(651)220-7178
Tracy.Smith <@t> Children'sHC.org
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