[Histonet] lung frozens

[Instrumedics]

Rita,
Please visit our  web site www.instrumedics.com to see how you can
snap-freeze lung tissue with the Gentle Jane device and cut paraffin-quality
frozen sections with the CryoJane Tape-Transfer system .

Click on the "gallery" to see CryoJane prepared frozen sections among them
lung frozens.

If you have any questions please contact us. We welcome your questions.

Bernice
----- Original Message ----- 
From: "Rita Angel" <RITA.ANGEL <@t> UC.EDU>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, January 05, 2004 2:46 PM
Subject: [Histonet] freezing mouse lungs


> Hi all,
>
> I was wondering if anyone could offer help in obtaining the best sections
> possible with frozen mouse lungs. How can I prepare the lungs and freeze
> them to get the best results? I know this is difficult tissue to get good
> sections on anyway, with all the air pockets. Is it possible to infuse
them
> with freezing embedding media?
>
> The lung sections I'm getting have a lot of holes, and at best, I'm only
> getting tissue from half of the lung samples while cutting. They were
> brought to me already frozen and embedded so I'm not sure how they were
> prepared.
>
> Thanks for your help,
>
> Rita Angel, HT
> University of Cincinnati
>

----- Original Message ----- 
From: <histonet-request <@t> lists.utsouthwestern.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, January 06, 2004 1:00 PM
Subject: Histonet Digest, Vol 2, Issue 5


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> Today's Topics:
>
>    1. RE: slide staining dishes? (Nancy Klemme)
>    2. uric acid fixation and processing (jamie0139 <@t> bellsouth.net)
>    3. RE: Epon resin (Mitchell (Jean A.))
>    4. RE: slide staining dishes? (Nancy Klemme)
>    5. freezing mouse lungs (Rita Angel)
>    6. Jon Opportunity: Histology Supervisor  (Dunikoski, Leonard  PhD)
>    7. automatic tissue processors (Atoska S. Gentry)
>    8. Microwave:To Steve Slap,Donna and Loralee (DMBCMP <@t> aol.com)
>    9. RNA DNA (im)possibilities (Hans Ooms)
>   10. Re: uric acid fixation and processing (Lee & Peggy Wenk)
>   11. plant histology (Evelyn Kaplan)
>   12. Re: RNA DNA (im)possibilities (David Walker)
>   13. More on uric acid (MTitford <@t> aol.com)
>


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