Fwd: Re: [Histonet] Advice.

[LaCinda Burchell]

>>> LaCinda Burchell 02/27/04 09:36AM >>>
Dear Ian,
 We dilute our antibodies with glyceol, aliquote them, and then store
them at -20F.  That way they are kept very cold without the potential
damage of ice crystal formation.  Once an aliquote is thawed it is never
put back into the freezer.  So far this method has served very well.   

LaCinda Burchell, BA, AS, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave.  CSC  K4/913
Madison, Wisconsin  53792

Phone: 608-262-3518
FAX:     608-263-3746

>>> Ian Montgomery <ian.montgomery <@t> bio.gla.ac.uk> 02/27/04 07:32AM >>>
         Antibody, store at 4C, do not freeze, stable for 1 year.
What's 
the current thinking. Are these antibodies affected by freezing? Or is
it 
just the company covering themselves against users thaw/freezing. In
the 
past I've just aliquoted and frozen anyway.
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 


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