[Histonet] endogenous peroxidases in liver sections

[Edmondson David (RBV) NHS Christie Tr]

Following on from Patsy,
we went on to using DAKO Envision detection system kit because of all the
Biotin blocking that we were doing.  And now things are clean.
3% Hydrogen Peroxide was a standard system for blocking endogenous
peroxidase.  Commercially available products are in the catalogues
And I remember Methanolic peroxide years ago, where we had 4ml of 30%
Hydrogen peroxide in 250ml of Methanol and blocked for thirty minutes!
Which seems a little aggressive/desparate.   
Dave
Manchester

-----Original Message-----
From: Cathy Malcontenti-Wilson [mailto:cmalc <@t> unimelb.edu.au]
Sent: 25 February 2004 22:36
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] endogenous peroxidases in liver sections


Hi,
Has anyone got any good method for blocking endogenous peroxidases in mouse 
liver sections? We seem to have a lot of red and white blood cells and 
plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit 
(mouse on mouse and universal kits). Some pearls of wisdom will be very 
much appreciated.
Thanks
Cat


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