[Histonet] Fixation of frozen tissue?

Joe Burdo burdo <@t> salk.edu
Wed Feb 25 17:20:01 CST 2004

 I have some dry ice frozen human brain samples that I would like to
section for IHC. We do not have a Cryostat in our lab, but we do have a
freezing microtome. Can the frozen brain chunks be fixed in 4% PF,
cryoprotected in 30% sucrose and then sectioned on the microtome for
free floating IHC? I have never cut tissue that has been fixed after
freezing, only tissue that has been fixed immediately after removal. 
Joseph Burdo, Ph.D.
Postdoctoral Associate
Cellular Neurobiology Laboratory
The Salk Institute for Biological Studies
10010 N. Torrey Pines Rd.
La Jolla, CA, 92037

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