Fwd: [Histonet] Isopentane

[Charles Scouten]

Isopentane can be chilled in a refrigeration device like the Clini RF, which also encloses it to prevent evaporation except when in use.  It is great for quick freezing tissue, does not require the hazard of liquid nitrogen, and does not freeze so fast it cracks the block.  See the following link for the Clini-RF

 http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=476401&catdesc=Histology+Equipment&CatThreeID=650&CatOneID=4&subcatdesc=Freezing+Devices&idsubcategory=187


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry
Sent: Tuesday, February 24, 2004 11:24 AM
To: Histonet
Subject: Fwd: Re: Fwd: [Histonet] Isopentane


Hello, I'm not aware of a procedure for quick freezing in isopentane alone, we quick freeze by immerging the sample in isopentane chilled by liquid nitrogen. Please will you share with me your protocol for quick freezing in isopentane. Thanks, Atoska


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>Date:  Tue, 24 Feb 2004 10:50:58 -0600
>From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
>To: <gentras <@t> vetmed.auburn.edu>
>Subject: Re: Fwd: [Histonet] Isopentane
>
>Isopentane is used to "quick freeze" frozen sections- can't liquid 
>nitrogen be use in its place? No specialty work (muscles, etc.) but 
>routine frozens.
>
> >>> "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu> 02/24/04 10:12AM
> >>>
>
>Hello, I don't believe isopentane can be substituted for liquid 
>nitrogen, they're usually used in conjunction. However, dry ice can be 
>substituted for liquid nitrogen. Best wishes. Atoska
>
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> >For Vinny- or anyone. I am trying to substitute liquid nitrogen for 
> >Isopentane in the frozen lab due to volatility. Could not access 
> >Histologic article from May regarding laboratory accident. Anyone 
> >have any other incidents?  Help would be appreciated.
> >
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>Atoska S. Gentry B.S., HT(ASCP)
>Research Assistant III
>Scott-Ritchey Research Center
>College of Veterinary Medicine
>Auburn University, AL  36849
>Phone# (334)844-5579  Fax# (334)844-5850
>
>
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Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 


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