[Histonet] ki67 or pcna (anti-mouse)

[Andi Kappeler]

Hi Daniel

I assume you want to stain (para)formaldehyde-fixed, paraffin-embedded mouse
tissue, right? Try rat-anti-mouse Ki-67, clone TEC-3 (DakoCytomation M
7249). We use it after HIER in a pressure cooker with citrate buffer, pH
6.0. Working dilution is 1:100 - 1:200, when using a 3-step protocol like
ABC, LSAB or similar. Visualization: use a x-a-rat Ig/Biotin secondary that
has been absorbed with mouse Ig to avoid demonstration of endogenous mouse
Ig in your tissue sections (e.g. DakoCytomation E 0468 at 1:300 - no, I
don't have any shares).
Good luck
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "DANIEL EBERHARD" <daniel.eberhard <@t> uni-bielefeld.de>
To: <histonet <@t> pathology.swmed.edu>
Sent: Monday, February 23, 2004 11:22 AM
Subject: [Histonet] ki67 or pcna (anti-mouse)


> Dear All,
> sorry if this question has been ask before,
> I´m searching for a ki67 or pcna (anti-mouse) antibody
> which works on pFA fixed tissue.
> Thanks for any hints.
> Daniel.
>
> -- 
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> Dr. Daniel Eberhard          =V=
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> Developmental Biology
> & Molecular Pathology
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> Graduate Programe on Pattern Formation
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> University of Bielefeld
> D 33501 Bielefeld/Germany
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