[Histonet] ki67 or pcna (anti-mouse)

Elizabeth Chlipala lizchlipala <@t> premierhistology.com
Mon Feb 23 10:48:22 CST 2004


Dako has a mouse anti Ki-67 (clone TEC-3) that works well in formalin
fixed paraffin embedded tissue.   Steam retrieval, primary antibody 1:50
for 30 minutes room temp, then Dako's Biotinylated Rabbit anti-rat at
1:200 for 30 minutes,  then streptavidin/HRP for 30  minutes, if you
find you need more amplification you can substitute the streptavidin/HRP
with Dako's Envision + rabbit, but you might need to change the dilution
of the primary.  Hope this helps.  


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DANIEL
Sent: Monday, February 23, 2004 3:23 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] ki67 or pcna (anti-mouse)

Dear All,
sorry if this question has been ask before,
I´m searching for a ki67 or pcna (anti-mouse) antibody
which works on pFA fixed tissue.
Thanks for any hints.

---------------------------  \"/ ---
Dr. Daniel Eberhard          =V=

Developmental Biology 
& Molecular Pathology 

Graduate Programe on Pattern Formation

University of Bielefeld
D 33501 Bielefeld/Germany

FAX: xx49(0)521-106-5654   (-)-(-)
---------------------------  \"/ ---

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