[Histonet] fragile tissue for immunohistochemisty

[Alexandra Allison de Sousa]

Hello!  I have been doing immunohistochemistry on some very fragile and 
large sections of ape brain.  The procedure requires so many transfers 
(washing and 2 pretreatments in addition to primary antibody, 
secondary...) that the tissue is shredded by the time it is stained and 
I am ready to mount it on a slide.  I assume that I could save the 
tissue some damage by mounting first before -- has anyone tried this? 
Would it work?  Any other ideas?


Thanks!
Alexandra