[Histonet] Toluidine Blue O

[Diane Gerjets]

Travis,
This is the procedure that we use in our lab. 1. Deparaffinize and 
rehydrate to water. 2. Stain in 1% Toluidine Blue O for 1 minute. ( Mix 
toluidine blue with distilled water ). 3. Tap water rinse. 4. Dehydrate, 
clear and mount. This has worked fine for us. We just use a mast cell control.
Diane Gerjets
Iowa Sate University
College of Veterinary Medicine
Ames, Iowa 50011