[Histonet] alkaline phosphatase

[Kathleen Spencer]

Yes, use plain DAB for your first AB and DAB nickel sulfate for your 
second. The procedure takes 4 days because you do them one at a time 
(with overnight incubation) You get brown for one and blue black for the 
other. Anyway, that is what we do.

Kathleen Spencer, HT
Lab Manager
UTHSC
On Wednesday, February 18, 2004, at 01:19  PM, Martin Weber wrote:

> Hello,
> I might need to do a dual staing procedure on paraformaldehyde fixed 
> fresh frozen sections. One marker will be stained with peroxidase 
> (DAB). As a second marker for IHC I read about alkaline phosphatase 
> (FastRed) which gives rise to a red color in addition to the brown 
> color of the peroxidase staining. To me this combination seems to be a 
> little bit unfortunate because the colors are sort of overlapping.
> Does anybody know of an alternative and complimentary method for dual 
> staining which might give rise to a different color other than red ? 
> Unfortunately due to special circumstances Immunofluorecence is not an 
> option.
> Any help is much appreciated. Thanks a lot !
>
> Martin Weber
>
> The Scripps Research Institute
> Dept. of Molecular and Experimental Medicine
> 10550 North Torey Pines Road
> La Jolla, 92037
> Phone: 858-784-8612
> Fax: 858-784-2030
> E-Mail: maweber <@t> scripps.edu
> Martin Weber M.D.
>
> Work address:
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> E-Mail: maweber <@t> scripps.edu
>
>
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