[Histonet] alkaline phosphatase

[Martin Weber]

Hello,
I might need to do a dual staing procedure on paraformaldehyde fixed 
fresh frozen sections. One marker will be stained with peroxidase (DAB). 
As a second marker for IHC I read about alkaline phosphatase (FastRed) 
which gives rise to a red color in addition to the brown color of the 
peroxidase staining. To me this combination seems to be a little bit 
unfortunate because the colors are sort of overlapping.
Does anybody know of an alternative and complimentary method for dual 
staining which might give rise to a different color other than red ? 
Unfortunately due to special circumstances Immunofluorecence is not an 
option.
Any help is much appreciated. Thanks a lot !

Martin Weber

The Scripps Research Institute
Dept. of Molecular and Experimental Medicine
10550 North Torey Pines Road
La Jolla, 92037
Phone: 858-784-8612
Fax: 858-784-2030
E-Mail: maweber <@t> scripps.edu
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Martin Weber M.D.

Work address:
The Scripps Research Institute
Department of Molecular and Experimental Medicine
10550 North Torrey Pines Road, Mail drop MEM 175
La Jolla, CA. 92037, U.S.A.
Phone at work: 001-858-784-8612
Fax at work: 001-858-784-2030
E-Mail: maweber <@t> scripps.edu 


Home address:
7265 Charmant Drive, Apt 628
San Diego, CA., 92122, U.S.A.
Phone at home: 001-858-452-0544