[Histonet] RE: VEGF in rat xenografts

[C.M. van der Loos]

Dear Nancy,
We have been using VEGF from Santa Cruz (A-20, sc-152) rabbit antibody successfully on human FFPE tissues (HIER with EDTA9.0, 1:200 overnight 4C, EnVision+ detection). A mamma tumor serves as a good positive control. The data sheet said there is a cross-reaction with other species including rat. 
Hope this helps.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  nperson211 <@t> comcast.net 
Date  Mon, 16 Feb 2004 21:12:19 +0000 
To  histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] VEGF in rat xenografts 
Histonetters,
Does anyone do VEGF IHC on FFPE sections of rats that have been implanted with human tumor cell lines?  If anyone does and would be willing to share their experiences, I would really appreciate it.
Thanks in advance (and with great hope!),
Nancy Lemke
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit, MI