[Histonet] vWf in rat kidneys - the answer

[antje.marcantonio <@t> pharma.novartis.com]

Hello Histonetters

vWf staining in kidneys is not a mystery anymore ! 
Adapting my protocol to the procedure I found in the report mentioned 
below , I got wonderful strong staining even in the glomeruli ! 
Pepsin was the trick ...(Proteinase K didn't help)
So now we run  two completely different protocols one for kidneys and one 
for lungs.

1: Kidney Int.  2000 Dec;58(6):2599-610. 

The vasculopathy of autosomal dominant polycystic kidney disease: insights 
from
animal models.

Arnaout MA.

Best regards,

Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland