[Histonet] GMS Stain

[Bill Sinai]

Perming solution contains thyoglycolate, if you purchase this in pure form
you then dilute it to about 1% and use it the same way as perming solution.
Just remember to dilute it in the fume cupboard as the smell is atrocious.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Weems,
Joyce
Sent: Tuesday, 17 February 2004 9:10 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] GMS Stain


I've had a few questions regarding the perm solution. I knew I had read
something somewhere - p445 Sheehan! She says to remove silver stains from
your hands with perm solution, so I thought it should work on tissue as
well. It has been quite a while ago. I believe I used it full strengh and
kept water close by to swish and dip until it looked just right. j:>)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bonner,
Janet
Sent: Tuesday, February 10, 2004 11:16 AM
To: 'Joyce Cline'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] GMS Stain



     To remove excess silver ,we dip the slides in 4% Chromic acid and rinse
in 1% sodium bisulfite, then check under the scope. If too light, start at
the silver step again.   Remember that generally a solution is first in the
process because it affects the remaining steps, either to enhance or remove!
                                   Janet

-----Original Message-----
From: Joyce Cline [mailto:jcline <@t> wchsys.org]
Sent: Thursday, February 05, 2004 3:39 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Fw: [Histonet] GMS Stain


I use a coplin jar of distilled water and I add two -three drops of clorox
and then dip the slides until the excess silver comes off. Rinse well in
water.
----- Original Message -----
From: "Poteete, Jacquie A." <japoteete <@t> saintfrancis.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 05, 2004 1:58 PM
Subject: [Histonet] GMS Stain


> I know there is something to use when the slides get left in the silver
> solution to long but have forgotten. Can someone refresh my memory?
> Thanks
>
>
>
>
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