[Histonet] GMS Stain

[Weems, Joyce]

I've had a few questions regarding the perm solution. I knew I had read
something somewhere - p445 Sheehan! She says to remove silver stains from
your hands with perm solution, so I thought it should work on tissue as
well. It has been quite a while ago. I believe I used it full strengh and
kept water close by to swish and dip until it looked just right. j:>)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bonner,
Janet
Sent: Tuesday, February 10, 2004 11:16 AM
To: 'Joyce Cline'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] GMS Stain



     To remove excess silver ,we dip the slides in 4% Chromic acid and rinse
in 1% sodium bisulfite, then check under the scope. If too light, start at
the silver step again.   Remember that generally a solution is first in the
process because it affects the remaining steps, either to enhance or remove!
                                   Janet

-----Original Message-----
From: Joyce Cline [mailto:jcline <@t> wchsys.org]
Sent: Thursday, February 05, 2004 3:39 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Fw: [Histonet] GMS Stain


I use a coplin jar of distilled water and I add two -three drops of clorox
and then dip the slides until the excess silver comes off. Rinse well in
water.
----- Original Message ----- 
From: "Poteete, Jacquie A." <japoteete <@t> saintfrancis.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 05, 2004 1:58 PM
Subject: [Histonet] GMS Stain


> I know there is something to use when the slides get left in the silver
> solution to long but have forgotten. Can someone refresh my memory?
> Thanks
>
>
>
>
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