[Histonet] Re: mouse perfusion

[Andrew Gray]

Hi Sharon,

I do perfusions on rats, and I place a surgical clamp over the heart to fix the needle (blunt, 18 gauge) in place.   The clamp I use resembles this: http://www.edmo.com/s14/42H.asp , but has a curved nose.   The needle is attached to a tube supplying the solutions; this keeps the liquid reservoirs well out of the way.   I use an electrical peristaltic pump to push the liquids through.

Best wishes

Andrew Gray
PhD student