[Histonet] control tissues

[Richard Cartun]

Yes, I frequently inject organisms into fresh lung tissue and then use
it as a positive control after formalin fixation and histological
processing.

Richard Cartun

>>> "Evelyn Kaplan" <ekaplan <@t> squ.edu.om> 02/09/04 10:49PM >>>
Hi Theresa,

Thanks for your reply but I am well familiar with the 'in-house' sytem
of
control tissues. I am more interested in inocultaing tissues, such as
fresh
placenta, with 'substances' to create a new control block. Just
wondering if
anyone has tried it!

Regards,
Evelyn

-----Original Message-----
From: Therersa Stegall [mailto:STEGTM <@t> samcstl.org] 
Sent: Tuesday, February 10, 2004 12:00 AM
To: ekaplan <@t> squ.edu.om 
Subject: Re: [Histonet] control tissues


Evelyn:  We make many of our own controls, using other cases that are
positive for the pathology tested for.  Our pathologists keep us
informed on + Helicobacter Pylori, and for other things, we use tissue
raided from surgicals (such as appendix for Trichrome, non-neoplastic
spleen for reticulum, liver for PAS).  Good liver for Fe controls is
hard to find, some Histonetters in England sent me a batch.  I think
you
could "raid" surgicals, and find nearly any tissue you need for
controls.  Our computer system allows a natural language search, so it
gets easier.  Peace,  Terre

>>> "Evelyn Kaplan" <ekaplan <@t> squ.edu.om> 2/8/2004 12:13:37 AM >>>


Good morning,

I realise this is a subject which has been previously discussed but I
wonder
if anyone has had experience of 'manufacturing' their own control
blocks? I
have a student about to start a project  and would apreciate any
protocols.

Regards,
Evelyn Kaplan,
Dept of Pathology,
College of Medicine and Health Sciences,
Sultan Qaboos University,
Oman


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