[Histonet] Dako ChemMate Envision kit

[Anila Syed]

Thank you very much for all the helpful suggestions and tips. I've decided
that it might be my aliquoted primary that has not been mixed properly and
is givign inconsistent results. So I am going to thaw the aliquots and remix
and realiquot. Is this a good idea?

Anila
----- Original Message ----- 
From: "Anila Syed" <syedab <@t> totalise.co.uk>
To: <histonet <@t> pathology.swmed.edu>
Sent: Monday, February 09, 2004 4:18 PM
Subject: [Histonet] Dako ChemMate Envision kit


> Hi all,
>
> I have been using the Dako Envision ChemMate kit for three weeks with no
> success. I wondered whether I was doing something obviously silly like
using
> the wrong pH. In the protocol it mentions to use the Dako buffer kit.
Could
> someone tell me whether it suddenly needs to change pH half way through
the
> protocol?
> I am using PBS at pH 7.4 made up from tablets.
>
> Thanks in advance
>
> Anila Syed
>
>
>
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