[Histonet] IHC ~ Tissue Falling Off Slides, drying procedure.slides

[Jones, Laura]

Hey Jeff!  We have tried many variations on drying as well.  Generally, we
cut the tissue, let it stand and drain/air dry at room temp for 0.5-1.0
hour, then place on the slide dryer at 65-70 for another hour, then place in
the slide oven at 80 to melt - usually about 20 minutes.  

We have also tried microwave drying. 

We have also dipped the slides (after the tissue is on them) into
Poly-L-Lysine.

Even slides that are cut on Saturday, dry all weekend and not retrieved
until Monday morning are troublesome.  


Dottie - we happen to have a few boxes of Superfrost Plus slides here from
our Cytology department and are cutting some test slides as I type this.
Thanks for the suggestion!