[Histonet] ER staining problem

[Luck, Greg D.]

Chris,

What clone(s) are your two ER ab's?

Greg Luck, BS, HT(ASCP)
Anatomic Pathology Supervisor
Deaconess Medical Center
800 W. 5th Ave
Spokane, WA 99204
Phone 509.473.7077
Fax 509.473.7133
luckg <@t> empirehealth.org



-----Original Message-----
From: Chris Evanish [mailto:CEvanish <@t> mont-hosp.com]
Sent: Wednesday, December 08, 2004 8:39 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ER staining problem


I need your help.
I use a Ventana ES and am having a real problem getting the ER antibody to
work. 
It was always a great result, however in the last 6 months or so it has
gotten worse to the point now where the stain is dead negative.
I've tried different slides, different pressure cookers, different retrieval
solution and even different ER antibodies. One from Ventana and one from
Zymed.
If you can help, or give me a few thing to try I would appreciate it.

        Thanks,
           Chris


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