[Histonet] IHC queries Bowman's layer and Descemet's Membrane
Marshall Terry Dr,
Terry.Marshall <@t> rothgen.nhs.uk
Wed Dec 8 10:57:06 CST 2004
I am constantly amazed at the requests for ipox to stain things which are perfectly well recognised without it. But, that is an aside.
I can never remember which b.... membrane is which, and only need the information every year or so.
Do you have a mnemonic?
(A mnemonic for the spelling of mnemonic would be useful too).
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Rotherham General Hospital
terry.marshall <@t> rothgen.nhs.uk
From: Paula Pierce [mailto:contact <@t> excaliburpathology.com]
Sent: 08 December 2004 16:32
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC queries Bowman's layer and Descemet's Membrane
I have been doing ophthalmic histology for 25 years. You do not need to do an immuno to demonstrate Descemet's membrane in the cornea. The simple PAS (periodic acid - Shiff's) stain will stain Descemet's a bright hot pink. It is routinely done with an H&E on recipient corneas from transplants. Bowman's, however, will not stain with PAS, but is the homogeneous layer right beneath the epithelium.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway
Moore, OK 73160
contact <@t> excaliburpathology.com
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