[Histonet] ER staining problem

[Stapf, Ross]

Are your slides cut fresh, or are they pre-cut days or weeks ahead?  ER
is one antibody some people have problems with if it is not cut fresh. 

Has something changed in the fixation? 

These are the only ideas I have on the subject right now.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS <@t> baylorhealth.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chris
Evanish
Sent: Wednesday, December 08, 2004 10:39 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ER staining problem


I need your help.
I use a Ventana ES and am having a real problem getting the ER antibody
to work.
It was always a great result, however in the last 6 months or so it has
gotten worse to the point now where the stain is dead negative. I've
tried different slides, different pressure cookers, different retrieval
solution and even different ER antibodies. One from Ventana and one from
Zymed. If you can help, or give me a few thing to try I would appreciate
it.

        Thanks,
           Chris


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