[Histonet] IHC queries Bowman's layer and Descemet's Membrane

[Paula Pierce]

Hello 
 
I have been doing ophthalmic histology for 25 years. You do not need to do an immuno to demonstrate Descemet's membrane in the cornea. The simple PAS (periodic acid - Shiff's) stain will stain Descemet's a bright hot pink. It is routinely done with an H&E on recipient corneas from transplants. Bowman's, however, will not stain with PAS, but is the homogeneous layer right beneath the epithelium.


Paula Pierce, HTL(ASCP)HT

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