[Histonet] Destaining DifQuik

Tony Henwood AnthonyH <@t> chw.edu.au
Thu Dec 2 16:46:08 CST 2004

To destain Romanowsky type stains, place in 1% acetic acid in 90% ethanol.

If the smears have been air-dried prior to DifQuik staining, then PAP
staining will be very dissapointing since the cells will be affected by
air-drying. There is no technique known to reverse this.


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Mary North [mailto:northma <@t> ohsu.edu] 
Sent: Friday, 3 December 2004 3:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Destaining DifQuik

Has anyone tried to destain DifQuik-stained cytology preps so that it
can be restained with PAP?
Mary North, HT(ASCP), HTL
Portland, OR
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