[Histonet] RE: fixation for RE: Masson's Trichrome on frozen heart sections

[GUTIERREZ, JUAN]

After sectioning we put the slide in a coplin jar with alcoholic formalin for 5 minutes, and proceed with the stain.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live free speech!


-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu] 
Sent: Thursday, December 02, 2004 11:20 AM
To: GUTIERREZ, JUAN; Histonet <@t> lists.utsouthwestern.edu
Subject: fixation for RE: Masson's Trichrome on frozen heart sections

Juan,

How do you fix fresh tissue frozen sections?  Bouins or NBF?  and fixation 
times?  If we were to do this stain, our tissues are NEVER prefixed before 
snap freezing.

A one step staining procedure makes a lot of sense when handling more 
fragile frozen sections.  I know one substitute light green with aniline 
blue, or vice versa - what ever one likes best.  This was discussed just a 
few months ago on Histonet.

At 09:48 AM 12/2/2004, you wrote:
>We use Gomori's trichrome on frozen sections.  It's a one step procedure 
>that gives you a faster answer.  Are you able to try alternatives to MTS?
>Good luck.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)