[Histonet] fixation for RE: Masson's Trichrome on frozen heart sections

[Gayle Callis]

Juan,

How do you fix fresh tissue frozen sections?  Bouins or NBF?  and fixation 
times?  If we were to do this stain, our tissues are NEVER prefixed before 
snap freezing.

A one step staining procedure makes a lot of sense when handling more 
fragile frozen sections.  I know one substitute light green with aniline 
blue, or vice versa - what ever one likes best.  This was discussed just a 
few months ago on Histonet.

At 09:48 AM 12/2/2004, you wrote:
>We use Gomori's trichrome on frozen sections.  It's a one step procedure 
>that gives you a faster answer.  Are you able to try alternatives to MTS?
>Good luck.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)