[Histonet] Re: Ammonia water pH and other bluing solutions[Sc anned]

[Kemlo Rogerson]

I think you are wrong, so I'll correct you, as you requested.

I thought the 'blue' state of haematin was, like litmus, the effect an
alkaline condition has on the siderphilic dye. Red, like litmus, denoted
acidic conditions. Damned if I know what colour (with the 'u') haematin goes
when neutral (bluey/ red?)

Running tap water takes longer, I concede, but good things are worth waiting
for and it's easier to make up; we rush too much I find. TIP: Never use
London water, been through too many kidneys!

-----Original Message-----
From: Jackie M O'Connor [mailto:Jackie.O'Connor <@t> abbott.com] 
Sent: 30 November 2004 19:09
To: Johnson, Teri
Cc: Histonet; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Ammonia water pH and other bluing
solutions[Scanned]

Anyone remember using saturated lithium carbonate?  I could probably use a 
little lithium right now . . . . . 

Correct me if I'm wrong (Pandora's box) but isn't the "bluing" step just 
bringing the slides BACK to a neutral pH after treating them with acid 
which makes them purple-ish?  I like the ammonia because it 'seems' to 
make the nuclei sharper and instantaneously - Li2Co3 and PBS seemed to 
make them "blah".(Perception is in the eye of the beholder)   Running tap 
water will make the same miracle happen, but it takes longer.






"Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
11/30/2004 12:51 PM

 
        To:     "Histonet" <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Re: Ammonia water pH and other bluing
solutions


Way back in the day, Brigati was using PBS on his immunostainer to blue
after the hematoxylin counterstain.  I have since used PBS to blue my
slides instead of ammonium hydroxide/water.  It's cheap, and very easy
on the sections, and I have plenty of it on hand.
 

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org


 
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