[Histonet] Immunohistochemistry for Vitamen E?

[Geoff McAuliffe]

If vitamin E is fat-soluble, it may be removed by the alcohols and 
xylenes during dehydration and clearing. If it survives embedding, I 
would not worry so much about it coming out during dewaxing. Why not try 
frozen sections of fixed material?

Geoff


pcfanti <@t> buffalo.edu wrote:

>I am trying to do immunohistochemistry for vitamen e in parrafin 
>embeded specimens. I'm worried that the vitamin e will be leached out 
>of the tissue during the deparrafinization step. I would appreciate 
>any help on this topic. Thank you.    
>Peter Fanti
>University at Buffalo 
>OB/GYN department
>pcfanti <@t> buffalo.edu
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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