[Histonet] MMP-1 immuno labelling of placenta

[SMITH,REBEKAH FELICIA]

I've been doing eNOS staining on placenta and I found it helpful 
to block in 1:10 30%peroxide to methanol for 1hr (to get rid of 
endogenous peroxidase-I know most people probably don't do it for 
that long though, but shorter times didn't help) and NaOH for 15 
min to deal with the blood cells staining.Good luck! I feel your 
pain:-)
                                     Rebekah Smith
On Mon Aug 16 11:30:51 EDT 2004, "Y. Wang" 
<ynwang <@t> u.washington.edu> wrote:

> Hello Louise,
> 
> Thank you for your suggestions.
> 1) Seeing as we could not go to the farm to collect the tissue 
> the tissue
> was kept on ice (~5 hours) while it was shipped to us and 
> immediately
> embedded. Do you think this is a problem?
> 2)We have not done any biotin blocking for these samples. Thank 
> you for
> suggesting that. We will try this next.
> 
> Thanks again
> Yak-Nam Wang
> 
> 
> On Mon, 16 Aug 2004, renton louise mrs wrote:
> 
>> 1. Was the tissue fresh when sampled? autolysis can alter IHC 
>> dramatically
>> 2.Placenta contains a lot of biotin...are you blocking 
>> adequately?
>> 
>> Regards
>> 
>> 
>> 
>> -----Original Message-----
>> From: "Y. Wang" <ynwang <@t> u.washington.edu>
>> To: Histonet <@t> lists.utsouthwestern.edu
>> Date: Fri, 13 Aug 2004 14:35:07 -0700 (PDT)
>> Subject: [Histonet] MMP-1 immuno labelling of placenta
>> 
>> Hello,
>> 
>> Has anyone had any experience immuno labelling placenta tissue 
>> with
>> anti-MMP1? We have tried this (cryosections with ABC kit with 
>> DAB) but are
>> getting alot of (what we assume to be) background and nothing 
>> that looks
>> like images we have seen on-line. Seeing as we have not had any 
>> experience
>> with placenta tissue we are questioning if we are took the 
>> tissue from the
>> correct place from the placenta (we were given a whole one which 
>> we took
>> random samples from).
>> 
>> Thank you for your help
>> Yak-Nam Wang
>> 
>> Senior Fellow
>> Department of Bioengineering
>> University of Washington
>> Box 357962
>> Seattle, WA 98195
>> 
>> Tel.: (206)-221-5873
>> Fax.: (206)-221-5874
>> 
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>> 
>> Louise Renton
>> Bone Research Unit
>> University of the Witwatersrand
>> Johannesburg
>> South Africa
>> .......so what IS the speed of dark?
>> 
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 



--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"