[Histonet] Re: doing immunos by hand (renton louise mrs)

[Shirley Powell]

As a matter of fact, yes.  :)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Todd
Sherman
Sent: Monday, August 23, 2004 2:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: doing immunos by hand (renton louise mrs)


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Well I must say, I'm impressed! How many people did you have performing
this many immunos? And how many different proteins were you trying to
detect on a "typical" run? With all of the slide handling and epitope
retrieval steps, not to mention blocking and counterstaining, I don't
see how this was done in an 8hr day. Did you all wear capes?

Regards,
Todd


Todd Sherman
President
HistoSoft Corporation
"Biology in a new form..."
Home: www.histosoft.com
Member Services: www.myhistosoft.com


histonet-request <@t> lists.utsouthwestern.edu wrote:
|
| Today's Topics:
|    4. Re: doing immunos by hand (renton louise mrs)
|
| ----------------------------------------------------------------------
|
| Message: 4
| Date: Mon, 23 Aug 2004 10:13:47 +0200
| From: "renton louise mrs" <rentonlf <@t> bru.wits.ac.za>
| Subject: Re: [Histonet] doing immunos by hand
| To: histonet <@t> lists.utsouthwestern.edu
| Cc: histonet <@t> lists.utsouthwestern.edu
| Message-ID: <1093248827.921a1660rentonlf <@t> bru.wits.ac.za>
| Content-Type: text/plain; charset="UTF-8"
|
| Doing immuno manually is entirely dependent on the volume. In a lab where
| I worked previously, we would do up to 400 slides by hand (we had a panel
| of over 100 diffent immunos to choose from). This was 8hrs HARD work. We
| had specially constructed perspex boxes to use as a moisture chamber, but
| in emergencies, large oblong Tupperware  containers with moistened filter
| paper or roller towel were also used. The slides would be raised from the
| paper by means of blank slides placed at right angles to the immuno slide
| so as to avoid  breaking nails when trying to overcome  the surface
| tensiion that would suck the slide down onto the surface.
|
| best regards
|
|
| -----Original Message-----
| From: Pathologyarts <@t> aol.com
| To: histonet <@t> lists.utsouthwestern.edu
| Date: Thu, 19 Aug 2004 13:59:05 EDT
| Subject: [Histonet] doing immunos by hand
|
| does anyone still do immunos by hand or are they all done with these $40
| billion automated machines?
|
| i would like to see what the options are as far as doing these things
| manually.
|
| primarily for derm specimens, HMB 45, S 100, KI 67.
|
|
| any help is appreciated,
| curt
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|
| Louise Renton
| Bone Research Unit
| University of the Witwatersrand
| Johannesburg
| South Africa
| .......so what IS the speed of dark?
|
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