[Histonet] urgent problem - cryoprotection

[Barbara Bublava]

sorry - I do mean embolism of fat not thrombosis :o)


----- Original Message ----- 
From: "Barbara Bublava" <barbara.bublava <@t> meduniwien.ac.at>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, August 23, 2004 8:09 AM
Subject: Re: [Histonet] urgent problem - cryoprotection


> Thank you very much for your help. My cryostat will soon be repaired and I
> decided to leave the specimes in succrose. But now there appears another
> problem. I will do oil red O with these specimens and my boss says now,
that
> the succrose and/or the O.C.T. is washing out the fat. He also says that
oil
> red O is not state of the art for diagnosing thrombosis of fat in
> lungtissue.
>
> I do not think so, but just to be shure I would be interested in your
> opinion.
>
> I know methods using Sudan Black or other dyes but they use the same
> principle as oil red O. The only "real" alternative I read about here in
> Histonet is using osmiumtetroxide and doing paraffinsections. But as I
> remember Osmiumtetroxide is not very healthy and has to be collected for
> disposal.
>
> Thank you all for your help!!!
>
> Barbara Bublava, Vienna
>
>
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