[Histonet] PAS with diastase

[MICHELE M]

Hi All,
 
We're looking for some help with our PAS with diastase stain.  For some reason, we can not get it to work. The PAS works fine but the diastase does not seem to be working.  We are using diastase of malt from Fisher and a 0.1M phosphate buffer at pH 6.0 from Polyscientific.  Our tech also tried using distilled water with the diastase instead of the buffer. The diastase solution is heated in a waterbath at 37 degrees for one hour. We have used liver, colon and esophagus tissue controls.  If anyone has any ideas what might be the problem, please let us know.
 
Thanks,
 
Michele

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