[Histonet] ?? about ferric chloride

lpwenk <@t> sbcglobal.net lpwenk <@t> sbcglobal.net
Mon Aug 16 19:13:27 CDT 2004

I think the answer is yes, but we have to do a little chemistry math.

The formula for ferric chloride anhydrous is FeCl3 (1 iron, 3 chlorines)

The formula for ferric chloride hexahydrate is FeCl3*6H2O (1 iron, 3
chlorines, and 6 waters)

The molecular weight (MW) of FeCl3 anhydrous is 162. When 6 waters is added
on, the MW of water is 6 x 18 = 108. So the molecular weight of FeCl3*6H2O
is 162 + 108 = 270.

So, out of the 270 MW of the hexahydrate, 108 is water, or about 40% of the
weight (108/240).

Hopefully, it would follow that, if you were to weigh out 10 g of the
anhydrous, all 10 grams would be ferric chloride.

However, if you weighed out 10 g of the hexahydrate, 40% would be water and
only 60% would be ferric chloride, or about 6 grams.

So to get the hexahydrate to equal anhydrous (for the ferric chloride), you
would have take the amount you need and multiply it by 270/162 = 1.67.

Or, if 10 g of anhydrous is needed, then multiply the 10 g x 1.67 = 16.7 g
of ferric chloride hexahydrate would equal 10 g of ferric chloride

Try that, and let us know how it works.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI

----- Original Message -----
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, August 16, 2004 2:31 PM
Subject: [Histonet] ?? about ferric chloride

I ordered by mistake ferric chloride hexahydrate instead of plain old
ferric chloride.    Will the waters make any difference in staining?   I
can always just try it and see what happens, but if it doesn't work I'd
be stuck with the open bottle of a chemical I can't use.   Anybody know?

Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital

Phone - 501.364.4240
Fax - 501.364.3912

The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
us immediately by replying to the message and deleting it from your
computer. Thank you.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list