[Histonet] Iron stain question

[Tony Henwood]

What were the smears fixed in?

If Carnoys or any fixative containing acid then iron will leach out.


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: WWmn916 <@t> aol.com [mailto:WWmn916 <@t> aol.com] 
Sent: Sunday, 15 August 2004 1:49 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Iron stain question


Hello again histonetters,
 
Can anyone give me any insight as to why some smear slides stained for iron

would not stains positive as it was expected they  would? Doctor say's the 
slides should have stained positive for iron because the  patient has
leukemia.  
The control stained positive as it should  have. Staining tech is sure all
her 
slides were exposed to solution.   Thanks for any help.
 
Deb King, HT(ASCP)
Sacramento, CA
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