[Histonet] Staining smears

Barbara Lentz Barbara_Lentz <@t> dahlchase.com
Fri Aug 13 11:30:05 CDT 2004

We have been running our smears on the Artisan stainer.  However, we
found the Wash solution was hemolyzing the RBC's.  We then started
fixing the smears with Methanol prior to staining.  When we brought the
Ventana on board for testing, we did the same thing.  We were getting
fine staining on both machines.  Barb

>>> "Therersa Stegall" <STEGTM <@t> samcstl.org> 08/13/04 11:26AM >>>
A question, if you will.  Does anyone out there know of a method to
stain peripheral and bone marrow smears on an automated stainer (we
recently obtained a Ventana Nexes).  I'm referrring to the routine
stains we usually run on several smears, along with the
paraffin-embedded marrow clot slides.  Right now, I'm running the
paraffin sections on the stainer, and doing the smears by hand for
of losing the smears on the machine.  Any ideas?  Peace,  Terre

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